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Bacteria Testing Microbiology Department May 13, 1999 The following results suggest that the ASAP solution is a broad spectrum anti-microbial agent. It is able to effectively stop the growth of, and in fact kill, a variety of bacteria. The ASAP Silver Supplement has been tested against the following organisms: Staphylococcus aureus (Pneumonia, eye infections, skin infections (boils, impetigo, cellulitis, and post-operative wound infections), toxic shock syndrome, meningitis, food poisoning, osteomyelitis, and many others) inhibited @ 2.5 ppm and killed @ 5 ppm. 1/22/99 BYU Report. Shigella boydii (Bacillary dysenteryńcharacterized by severe cramping abdominal pain and bloody diarrhea) inhibited @ 1.25 ppm and killed @ 2.5 ppm. 1/22/99 BYU Report. Salmonella arizona (Food poisoning, etc.) inhibited @ 2.5 ppm and killed @ 5 ppm. 1/28/99 BYU Report. Salmonella typhimurium (Food poisoning and enteric fever) inhibited and killed at a concentration of 2.5 ppm. 6/7/99 BYU Report. E. coli (Food poisoning, urinary tract infections, travelerķs diarrhea, diarrhea in infants, respiratory tract infections, and wound infections) inhibited and killed @ 2.5 ppm. 1/22/99 BYU Report. Haemophilus influenzae (Otitis media (ear infection), pneumonia, meningitis, throat and sinus infections (including epiglottitis in children and sinusitis), and suppurative arthritis in children) inhibited and killed @ 1.25 ppm. 1/22/99 BYU Report. Enterobacter aerogenes ( wound infections, urinary tract infections, bacteremia, and meningitis) inhibited and killed at a concentration of 2.5 ppm. 6/7/99 BYU Report. Enterobacter cloacae ( causes ilnesses similar to the E. aerogenes) inhibited and killed at a concentration of 5 ppm. 6/7/99 BYU Report. Klebsiella pneumoniae (lower respiratory tract infections, nosocomial infections (infections spread in hospitals), urinary tract and wound infections, and bacteremia) inhibited and killed @ 2.5 ppm. 1/28/99 BYU Report. Klebsiella oxytoca, (Similar to those infections caused by K. pneumoniae) inhibited and killed at a concentration of 2.5 ppm. 6/7/99 BYU Report. Pseudomonas aeruginosa (severe burn and wound infections, keratitis, pneumonia, meningitis, nosocomial infections, urinary tract infections, etc.) inhibited @ 2.5 ppm and killed @ 5 ppm. 1/22/99 BYU Report. Streptococcus pneumoniae (pneumonia, meningitis, sinusitis, otitis media (ear infection) inhibited @ 2.5 ppm and killed @ 5 ppm. 4/21/99 BYU Report. Streptococcus pyogenes (skin infections, upper respiratory infections (i.e. strep throat) impetigo, hospital-acquired infections, scarlet fever, etc.) inhibited and killed @ 1.25 ppm. 1/22/99 BYU Report. Streptococcus faecalis (Urinary tract infections, endocarditis, wound infections, etc.) inhibited @ 2.5 ppm and killed @ 5 ppm. 1/22/99 BYU Report. Streptococcus mutans (A major cause dental plaque and tooth decay etc.) inhibited and killed @ 5 ppm. 2/3/99 BYU Report. Streptococcus gordonii (Tooth decay, also implicated in infective endocarditis-an infection of the heart valves) inhibited and killed @ 5 ppm. BYU Report 2/12/99. David A. Revelli Dr. Ron W. Leavitt, Ph.D. |
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Non - Toxicity Text Work Summary In order to insure not only the best product, but also a safe product, an international and independent laboratory was hired to do a toxicology study on the ASAP Solution. The test, called an LD-50 test, was performed in accordance with the guidelines of the Federal Hazardous Substances Act (FHSA) Regulations, 16 CFR 1500. In the test work, the ASAP Solution was given to a number of both male and female test rats. The amount of ASAP Solution given to the rats was 5g/kg, or the equivalent of a 200 pound man taking 192 teaspoons of about 4 full 8 ounce bottles of the ASAP 14ppm solution at one time (the normal adult dosage is one or two teaspoons/day). As a result of the test work, the independent laboratory made the following conclusion, Under the conditions of this study, there was no mortality or significant evidence of toxicity observed in the rats. The test article (ASAP Solution) would not be considered toxic at a dose of 5g/kg by oral route in the rat. |
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EColi Testing BACTERIA TESTING Microbiology Department October 4, 1999 To: American Silver, LLC Minimum Inhibitory Concentration (MIC) tests were performed with E.coli serotype 0157:H7 (more popularly known as the Jack-in-the-box bacteria), which has been associated with hemorrhagic colitis due to ingestion of contaminated food. Preliminary results showed that the ASAP solution inhibited growth at a concentration of 2.5 ppm and killed the bacteria at a concentration of 5.0 ppm. David A. Revelli |
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Water Treatment Tests WATER TREATMENT TESTS FROM: Bill Dodds TO: Gerard Yates RE: ASAP Solution water tests Procedure: I collected 500 ml of raw (Provo River water) sample and inoculated the sample with 2 loops (3mm) of Klebsiella oxtyoca. Using the membrane filter technique for total coliform, five 100 ml of aliquots of the sample were filtered. Number 1 was spiked with a .10 mg/l concentration of silver solution. Number 2 was spiked with a .20 mg/l concentration of silver solution. Numbers 3 and 4 were each spiked with a .50 mg/l concentration of silver solution. A positive control of 100 ml was used without silver addition. A 1.5 minute contact time was allowed on sample 1 before filtering. A 2 minute contact time was used on sample 2. A 10 minute contact time was used for samples 3 and 4. The filters were incubated at 35° C for 24 hours. RESULTS: The positive control = 705 cfu/100 ml. Samples 1 thru 4 contained 0 cfu/100 ml. William J. Dodds TO: American Silver CONCLUSION: ASAP solution effectively killed all colony forming units (bacteria) in a spiked raw river water sample containing a 0.10 ppm concentration of ASAP solution in 1.5 minutes of contact. Note: The ASAP solution was not removed from the water sample prior to filtration. Therefore the ASAP solution residual on the membrane filter may have inhibited growth during incubation. Gerard Yates |
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Yeast Testing Growth Inhibition of S. cerevisiae var. Montrachet in 0 to 15 ppm ASAP Solution. Jason Henrie, UC Davis, 4/13/99 Results: The ASAP Solution definitely inhibits the growth of yeast and the difference between treatments is statistically significant. The maximum growth rate and the final population for all but the 15 ppm treatment are the same, so inhibition is due to a delay in the onset of growth. The 15 ppm treatment presents a more interesting situation in that there is essentially no growth. It is important to note that the ASAP Solution was applied in only one dose and that in a real-world situation it will be applied multiple times. It is evident that a single 10 ppm application could prevent the further growth of a small population of yeast for 24 hours, allowing oneķs immune system time to respond. Even more, multiple 10 ppm applications could conceivably prevent the growth of yeast indefinitely. |
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Infection laboratory tests: As the data suggests, the ASAP solution may be effective in laboratory in vitro tests against the bacteria tested. Microbiology Department October 15, 1999 To: American Silver, LLC The following page contains data from seven strains of potentially pathogenic bacteria. They are the same strains that have been tested in Minimum Inhibitory Concentration tests (MIC) against American Silver's ASAP solution. This data contains, along with the data on the ASAP solution, MIC's performed on each bacteria in comparison to representative of five different classes of antibiotics. These tests were performed to ascertain the relative antimicrobial activity of the ASAP solution when compared to certain antibiotics... Each antibiotic tested has an effective niche. The ASAP solution was effective in this laboratory in vitro environment in many instances also. This is where the ASAP solution data comes in-although the ASAP solution may not have inhibited a strain of bacteria at a lower concentration than a given antibiotic, it did inhibit many strains of bacteria tested. Minimum Inhibitory Concentration of Antibiotics from Five Different Classes versus ASAP Solution performed by D. Revelli, Brigham Young University
The average was taken from all data points to obtain the average minimum inhibitory concentration for an antibiotic. Then a standard deviation was determined to give an error. All concentrations were calculated in parts per million (ug/ml). The abbreviation "no inh," stands for "No inhibition of growth." And">" means that the measurement of the bacteriocidal concentration was beyond the limits of the test. Information is listed as "MIC (level at which bacteria was inhibited)/MBC (level at which bacteria were killed)/number of tests performed." David A. Revelli Dr. Ron W. Leavitt, Ph.D. |
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ASAP vs. Other Silvers BACTERIA TESTING Microbiology Department June 18, 1999 TO: American Silver, LLC From: David Revelli We have tested and compared the activity of American Silver's ASAP solution against three commercial colloidal silver solutions using the Minimum Inhibitory Concentration test (MIC) and the Minimum Bactericidal Concentration (MBC) test. The data obtained suggests that the ASAP solution is at least two to three times more effective depending on the bacteria tested than the commercial colloidal silver products tested. David A. Revelli |
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Scientific Backing Summary Finally, A Silver Solution With Scientific Backing! Every supplement user dreams of a product that: is patented; has filed for drug approval; has filed with the EPA; has numerous universities and other independent laboratories backing its product with tests and information; has head to head comparisons against prescription antibiotics; has safety data; has done testing against bacteria and yeasts; and has even been tested for use in water treatment! That product is now available to you! The one and only ASAP Solution. Patented: ASAP is the only silver product so new and effective that it was deemed a new product, and therefore approved for patent by the United States Patent Agency. ASAP has the only two patent approvals on a silver solution product in the last 80 years. The patent has also been filed internationally, as well as specifically in Japan. FDA: ASAP Solution is the only silver solution product that has been successfully filed with the FDA for testing. EPA: The ASAP Solution has just passed the US-EPA tests for water treatment and also as a hard surface disinfectant. Tested under worst-case conditions, the ASAP Solution was able to eradicate 177 of 180 strains of Salmonella choleraesuis in less than five minutes. In the testing, the ASAP Solution was also able to kill 179 of 180 strains of both Staphylococcus aureus and Pseudomonas areuginosa in less than 15 minutes. One of the most notable facts of the testing was that the ASAP Solution passed all of the EPA tests at a concentration of just 4.4 ppm, a dilution of 56% from the 10 ppm supplement currently being sold worldwide. One third of the EPA tests were successfully completed with product that was three years old, which has also proven the stability and long-term storage capabilities of the ASAP Solution. The ASAP Solution was able to kill every single strain of the bacteria that it was tested against in the EPA tests, but not all were killed in the allotted time. In total, the ASAP Solution was able to kill 536 of 540 strains of bacteria at the diluted 4.4 ppm level, in just 15 minutes of contact time. Testing: There are numerous independent universities and private laboratories that have been and are currently doing test work on the ASAP Solution. Test work completed so far includes: antibiotic test comparisons; bacteria work; yeast work; safety and toxicity data; independent tests against other silver products; water treatment tests, etc. Humanitarian Aid: The ASAP Solution is the only silver solution approved by an international health consortium as an antibiotic alternative, for use in its hospitals and also in water treatment units in Africa, South America, and Asia. |
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Why Use the ASAP Silver Solution? Historically Used on and in the Body In ancient Greece and Rome, people used metallic silver containers to keep liquids fresh. American settlers and miners used silver dollars in their water barrels to kill bacteria, to help make the water drinkable. Early farmers also placed silver bars and coins in their milk containers to delay its spoiling. It has also been stated that the reason many members of the royal houses never suffered from the same illnesses that the peasants suffered from, was that royalty ate and drank from silver plates and cups, and their food was kept in silver containers, which have been proven to kill bacteria. During the plague in Europe, children from wealthy families were given silver spoons to suck on, hence came the saying, Born with a silver spoon in your mouth. Metallic silver has been used for centuries as a natural antibacterial mineral. What Form Of Silver Product Should I Use? Over the years silver has been used in many forms. Some types of silver products are more benign in nature and some much more caustic. The more caustic forms of silver that are used are chemical, not metallic, such as silver nitrate or silver salts. In the past, silver salts have been used for various medical conditions and also as an anti-bacterial for medical purposes. Some of the silver salts have been used and abused at unheard of levels, even as high as 305,000 ppm (parts per million), used without dilution. That is over 30% straight silver in chemical form. Silver salts can stain and burn the skin. They are even used to cauterize wounds. The more benign forms of silver have also been used for many years to kill bacteria and to treat medical problems. The more benign forms of silver are products that use silver in the form of a metal, where the metal has been broken up in very small, or submicron size particles, and then suspended in highly purified water. With this form of silver, the user can obtain the bacteria - killing power of silver and yet not suffer any of the side effects associated with the stronger silver chemicals. What is the ASAP Solution? The ASAP Solution is a silver solution, that has a patented process of making the bacteria- killing - yet safe - small particles of silver. The ASAP SolutionĘ is the only patented silver product of this kind in the world today. The ASAP Solution is so effective at killing bacteria that even after being diluted to 1/200 of the original 10 ppm level, the ASAP Solution was still able to kill all the bacteria in raw river water in less than 20 minutes. The ASAP Solution has also been proven very safe to use. Even when tested at 200 times the normal adult dosage, the ASAP Solution was found to be completely non-toxic. The ASAP SolutionĘ is the only silver solution of this type on the market that has been proven both safe for use and very effective at killing bacteria. |
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Newspaper Article #01 The following refers to in vitro laboratory testing in a controlled environment and not on or in the human body. By Lois M. Collins ...Researchers in Brigham Young University's department of microbiology were asked to test the antimicrobial activity of the ASAP Solution, one of several silver solutions available as unregulated natural supplements. Silver is "colloidal" when it is suspended in VERY small amounts in liquid. Silver in various forms has been used for centuries as an antimicrobial agent. In the 1800s and early 1900s, people put silver coins in their water barrels to kill microbes and make the water potable. A silver nitrate ointment is applied to the eyes of newborn babies to prevent certain eye problems. And silver suphadiazine is regularly used to treat burn wounds. Use of colloidal silver, once common, faded with the advent of antibiotics... According to the study, conducted by BYU's David A. Revelli, microbiologist, and Ron W. Leavitt. The study compared the ASAP Solution and five classes of antibiotics: the tetracyclines, fluorinated quinolones (Ofloxacin), the penicillins, the cephalosporins (Cefaperazone) and the macrolides (Erythromycin). Both the silver and antibiotics were tested on a variety of microorganisms, including streptococcuses, pneumonia, E. coli, salmonella, shigella and others. According to the study, the solution "exhibits an equal or broader spectrum of activity than any one antibiotic tested." Where each antibiotic was effective against specific susceptible organisms, the solution "is equally effective" against both gram positive and gram negative organisms. "The data suggests that with the low toxicity associated with essential silver, in general, and the broad spectrum of antimicrobial activity of this silver preparation, this preparation may be effectively used as an alternative to antibiotics," Revelli and Leavitt wrote. Dr. Dianne Farley-Jones, a family practitioner, recommends the solution to her patients for external problems. She hasn't used it internally much, though she said it works quite well for ear infections. "With any kind of abrasion or skin problem, it works really well and really fast. And it seems to have an anti-inflammatory effect, though that hasn't been proven." The silver solution also seems to have an antiviral effect, Farley-Jones said, though data hasn't been collected to prove it. She's used different brands at different times but hadn't recommended the solution until she saw the BYU research data. Now she encourages patients to use it as a nasal rinse for sinus infection or to spray their throats if they feel like they're getting a viral sore throat. She doesn't expect it to replace antibiotics. For one thing, just as people developed resistance to the antibiotics, "we don't know if there's some mechanism of resistance and people can develop it to silver, as well. But I am glad we have this tool. Using the same formulation (ASAP) the (BYU) tests were done on, I've used it enough and had good results." *** This product is not intended to diagnose, treat, cure, or prevent any disease. It is not intended to replace any FDA approved or physician recommended treatments of any kind. |
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Newspaper Article #02 ASAP solution tested extensively By ANN POTEMPA UTAH COUNTY - Dr. Ron Leavitt said BYU scientists are studying a new silver solution to verify anecdotes that say the solution is making people feel better. The silver solution also seems to have an antiviral effect, Farley-Jones said, though data hasn't been collected to prove it. She's used different brands at different times but hadn't recommended the solution until she saw the BYU research data. Now she encourages patients to use it as a nasal rinse for sinus infection or to spray their throats if they feel like they're getting a viral sore throat. She doesn't expect it to replace antibiotics. For one thing, just as people developed resistance to the antibiotics, "we don't know if there's some mechanism of resistance and people can develop it to silver, as well. But I am glad we have this tool. Using the same formulation (ASAP) the (BYU) tests were done on, I've used it enough and had good results." *** This product is not intended to diagnose, treat, cure, or prevent any disease. It is not intended to replace any FDA approved or physician recommended treatments of any kind. |
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